2. Epigenetics PI3K/Akt/mTOR Autophagy
  3. AMPK Autophagy Mitophagy
  4. Metformin hydrochloride

Metformin hydrochloride  (Synonyms: 盐酸二甲双胍; 1,1-Dimethylbiguanide hydrochloride)

目录号: HY-17471A 纯度: 99.92%
COA 产品使用指南

摩尔计算器

Metformin hydrochloride (1,1-Dimethylbiguanide hydrochloride) 抑制肝脏中的线粒体呼吸链，导致 AMPK 活化，增强胰岛素敏感性，可用于 2 型糖尿病的研究。Metformin hydrochloride 可以透过血脑屏障，诱导自噬 (autophagy)。

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Metformin hydrochloride Chemical Structure

Metformin hydrochloride Chemical Structure

CAS No. : 1115-70-4

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规格 价格 是否有货 数量
10 mM * 1 mL in DMSO ￥550
In-stock
5 mg ￥312
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10 mg ￥500
In-stock
25 mg ￥800
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100 mg   询价  

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Customer Review

Other Forms of Metformin hydrochloride:

Metformin hydrochloride 相关抗体

Top Publications Citing Use of Products

MCE 顾客使用本产品发表的 121 篇科研文献

WB
IHC
Cell Viability Assay

    Metformin hydrochloride purchased from MCE. Usage Cited in: Redox Biol. 2023 Jun 15, 102786.

    Metformin hydrochloride (Met; 3, 6 mM; 30 min) inhibits MGO-induced the increased expression of Bax and caspase-3 cleavage and decreased expression of Bcl-2 in ARPE-19 cells.

    Metformin hydrochloride purchased from MCE. Usage Cited in: Redox Biol. 2023 Jun 15, 102786.

    Metformin hydrochloride (Metformin; 2, 6, 20 mM; 30 min) attenuates MGO-induced cell death in a concentration-dependent manner in ARPE-19 cells.

    Metformin hydrochloride purchased from MCE. Usage Cited in: Endocrinology. 2018 May 1;159(5):2008-2021.  [Abstract]

    Male C57BL/6 mice are intragastrically treated with Metformin or placebo at 3 mg/kg/day for 10 weeks in the presence of HFD after 10-week-HFD feeding and then sacrificed for sample collection. Western blot analysis of AMPKα1, AMPK, p-AMPK, SREBP1 and FAS.

    Metformin hydrochloride purchased from MCE. Usage Cited in: Mol Cell Proteomics. 2017 Jul;16(7):1324-1334.  [Abstract]

    To further validate the MS quantification results, Western blot analysis is carried out using an anti-H3K36 dimethylation antibody. Consistent with the mass spectrometric data, H3K36 dimethylation was elevated in DIO mouse livers, whereas Metformin treatment can significantly decrease histone H3K36 dimethylation in DIO mice to a level close to that of the chow-fed control mice.

    Metformin hydrochloride purchased from MCE. Usage Cited in: Mol Oncol. 2017 Aug;11(8):1035-1049.  [Abstract]

    Co-treatment of Metformin and Ribociclib induces cell death in Hep3B cells. Cells are exposed to Ribociclib at 25 μM and/or Metformin at 10 mM for 72 h.

    Metformin hydrochloride purchased from MCE. Usage Cited in: Mol Oncol. 2017 Oct;11(10):1475-1492.  [Abstract]

    (A) IHC staining of N-cadherin and E-cadherin in the pancreas from KPC mice treated with vehicle, Metformin, or KRIBB11. (B) Masson's trichrome staining and IHC staining of α-SMA in pancreatic tissues from mice treated with vehicle, Metformin, or KRIBB11.

    Metformin hydrochloride purchased from MCE. Usage Cited in: Mol Oncol. 2017 Oct;11(10):1475-1492.  [Abstract]

    MiaPaCa-2 cells are treated with a gradient concentration of Metformin for 24 h, and then, western blotting is performed to show the activation of p-AMPK and the suppression of HSF1 and HSP70.

    Metformin hydrochloride purchased from MCE. Usage Cited in: J Cell Mol Med. 2017 Dec;21(12):3322-3336.  [Abstract]

    Metformin prevents the loss of tight junction (TJ) proteins after SCI. Representative Western blots and quantification data of Occludin, Claudin-5, ZO-1 and β-actin at 3 day after injury.

    Metformin hydrochloride purchased from MCE. Usage Cited in: Biochem Pharmacol. 2017 Aug 15;138:49-60.  [Abstract]

    PP5 overexpression suppresses AMPK-Thr172 phosphorylation induced by AMPK activators, AICAR and metformin. Hep3B cells are transfected with indicated plasmids and treated with AICAR (2 mM; 3 h) or Metformin (3 mM; 16 h) to simulate AMPK phosphorylation.

    Metformin hydrochloride purchased from MCE. Usage Cited in: Mol Neurobiol. 2017 Jul;54(5):3327-3341.  [Abstract]

    Met attenuates apoptosis caused by traumatic spinal cord injury in rat. a, b Representative western blots and quantification data of cleaved caspase 3 and β-actin in each group rats. c, d Representative western blots and quantification data of Bax, Bcl-2, and β-actin in each group rats.

    Metformin hydrochloride purchased from MCE. Usage Cited in: Cell Physiol Biochem. 2017;44(4):1381-1395.  [Abstract]

    U87 cells are treated with Met alone or in the presence of isogambogenic acid for 24 h. The expression levels of phosphorylated AMPK, mTOR and 4E-BP1 are assessed by western blotting, with total AMPK, mTOR and 4E-BP1 used as the internal controls.

    Metformin hydrochloride purchased from MCE. Usage Cited in: Front Immunol. 2016 Dec 12;7:597.  [Abstract]

    Metformin increases adenosine triphosphate-induced inflammasome activation in bone marrow-derived macrophages. (A) Cells are treated. Indicated protein levels in both cell lysates and supernatants are evaluated by western blotting. β-Tubulin is used as a loading control for cell lysates. (B–D) The quantitative analyses of the active caspase-1p10 (B), mature interleukin-1β (C), and HMGB1 (D) levels in the supernatants (A) are shown.

    Metformin hydrochloride purchased from MCE. Usage Cited in: Front Pharmacol. 2016 Oct 20;7:390.  [Abstract]

    Metformin, an AMPK agonist, counteracts the effect of Piperine on suppressing ATP-induced AMPK activation and inflammatory mediator release. J774A.1 cells are pre-treated with 80 μM Piperine and BMDMs are pre-treated with 160 μM Piperine for 4 h. Without being washed out of Piperine, these cells are primed with LPS (500 ng/mL) for 4 h. Next, the cells are treated with Metformin (1 mM) for 1 h (in the absence of Piperine and LPS). Finally, in the presence of Metformin, the BMDMs are stimulated wi

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    NUAK1 NUAK2 AMPK AMPK α1β1γ1 AMPK α2β1γ1 AMPK α1β2γ1 AMPK α2β2γ1

    • 生物活性

    • 实验参考方法

    • 纯度 & 产品资料

    • 参考文献

    生物活性

    Metformin hydrochloride (1,1-Dimethylbiguanide hydrochloride) inhibits the mitochondrial respiratory chain in the liver, leading to activation of AMPK, enhancing insulin sensitivity for type 2 diabetes research. Metformin hydrochloride triggers autophagy[1].

    IC50 & Target[2]

    AMPK

     

    体外研究
    (In Vitro)

    Metformin hydrochloride (1,1-Dimethylbiguanide hydrochloride) inhibits proliferation of ESCs in a concentration-dependent manner. The IC50 is 2.45 mM for A-ESCs and 7.87 mM for N-ESCs. Metformin shows pronounced effects on activation of AMPK signaling in A-ESCs from secretory phase than in cells from proliferative phase[2].
    Metformin hydrochloride (0-500 μM) decreases glycogen synthesis in a dose-dependent manner with an IC50 value of 196.5 μM in cultured rat hepatocytes[3].
    Metformin hydrochloride shows cell viability and cytotoxic effects on PC-3 cells with IC50 of 5 mM[4].

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.

    Metformin hydrochloride 相关抗体:
    体内研究
    (In Vivo)

    Metformin hydrochloride (1,1-Dimethylbiguanide hydrochloride; 100 mg/kg, p.o.) alone, and metformin (25, 50, 100 mg/kg) with NSC 37745 groups attenuates myocyte necrosis through histopathological analysis[1].

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.
    Clinical Trial
    分子量

    165.62

    Formula

    C4H12ClN5
    CAS 号
    性状

    固体

    颜色

    White to off-white

    中文名称

    盐酸二甲双胍；甲福明；降糖片
    运输条件

    Room temperature in continental US; may vary elsewhere.
    储存方式

    4°C, sealed storage, away from moisture

    *In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

    溶解性数据
    细胞实验： 

    H2O 中的溶解度 : ≥ 100 mg/mL (603.79 mM)

    DMSO 中的溶解度 : ≥ 1.7 mg/mL (10.26 mM; 吸湿的 DMSO 对产品的溶解度有显著影响，请使用新开封的 DMSO)

    * "≥" means soluble, but saturation unknown.

    配制储备液
    浓度 溶剂体积 质量 1 mg 5 mg 10 mg
    1 mM 6.0379 mL 30.1896 mL 60.3792 mL
    5 mM 1.2076 mL 6.0379 mL 12.0758 mL
    查看完整储备液配制表

    * 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
    储备液的保存方式和期限：-80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)。-80°C储存时，请在6个月内使用，-20°C储存时，请在1个月内使用。

    * 备注：如您选择水作为储备液，请稀释至工作液后，再用 0.22 μm 的滤膜过滤除菌后使用。

    • 摩尔计算器

    • 稀释计算器

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

    质量
    =
    浓度
    ×
    体积
    ×
    分子量 *

    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    This equation is commonly abbreviated as: C1V1 = C2V2

    浓度 (start)

    C1

    ×
    体积 (start)

    V1

    =
    浓度 (final)

    C2

    ×
    体积 (final)

    V2

    动物实验：

    请根据您的 实验动物和给药方式 选择适当的溶解方案。

    以下溶解方案都请先按照 In Vitro 方式配制澄清的储备液，再依次添加助溶剂：
    ——为保证实验结果的可靠性，澄清的储备液可以根据储存条件，适当保存；体内实验的工作液，建议您现用现配，当天使用；
    以下溶剂前显示的百分比是指该溶剂在您配制终溶液中的体积占比；如在配制过程中出现沉淀、析出现象，可以通过加热和/或超声的方式助溶

    • 方案 一

      请依序添加每种溶剂： 5% DMSO    40% PEG300    5% Tween-80    50% Saline

      Solubility: ≥ 3 mg/mL (18.11 mM); 澄清溶液

    • 方案 二

      请依序添加每种溶剂： 5% DMSO    95% (20% SBE-β-CD in Saline)

      Solubility: ≥ 3 mg/mL (18.11 mM); 澄清溶液

    以下溶解方案，请直接配制工作液。建议现用现配，在短期内尽快用完。 以下溶剂前显示的百分比是指该溶剂在您配制终溶液中的体积占比； 如在配制过程中出现沉淀、析出现象，可以通过加热和/或超声的方式助溶。

    • 方案 一

      请依序添加每种溶剂： PBS

      Solubility: 100 mg/mL (603.79 mM); 澄清溶液; 超声助溶

    动物溶解方案计算器
    请输入动物实验的基本信息：

    给药剂量

    mg/kg

    动物的平均体重

    g

    每只动物的给药体积

    μL

    动物数量

    只
    由于实验过程有损耗，建议您多配一只动物的量
    计算结果
    工作液所需浓度 : mg/mL
    该产品水溶性佳，请具体参考实测 水 / PBS / Saline 中的溶解度数据。
    您所需的储备液浓度超过该产品的实测溶解度，如有需要，请与 MCE 中国技术支持联系。
    纯度 & 产品资料

    纯度: 99.92%

    COA (283 KB) HNMR (224 KB) LCMS (95 KB)

    产品使用指南 (1538 KB)
    参考文献
    Cell Assay
    [2]

    ESCs are plated in 96-well plates at a concentration of 1×103cells/well. After attachment, cells are treated with different doses of metformin/compound C for 0 min, 15 min, 1 h, and 24 h. MTT assays are performed as described previously. In brief, MTT (5 mg/mL) is added to the 96-well plates at a volume of 10 μL/well, and the plates are incubated for 4 h. The MTT reaction is terminated by removal of the culture medium containing MTT, and 100 μL DMSO per well are added and incubated at RT on a shaker for 10 min to ensure that the crystals had dissolved sufficiently. Absorbance values are measured at 595 nm. Cell proliferation (percentage of control) is calculated as follows: absorbance (experimental group)/absorbance (control group). Cell proliferation inhibition (percentage of control) is calculated as follows: 100%−cell proliferation (percentage of control). Each experiment is performed in duplicate and repeated six times to assess result consistency.

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.
    Animal Administration
    [1]

    The animals are randomized into six groups consisting of six rats each. Rats in group 1 (control) receives a subcutaneous injection of physiological saline (0.5 mL) and are left untreated for the entire experimental period. Rats in group 2 receives an oral administration of metformin (100 mg/kg; twice daily) for 2 days and are subcutaneously injected with saline at an interval of 24 h for 2 consecutive days. Rats in group 3 (MI control) receives an oral administration of saline (twice daily) for 2 days and are sc injected with NSC 37745 (100 mg/kg) daily for 2 consecutive days at an interval of 24 h. Rats in groups 4 to 6 are treated with metformin at 25, 50, and 100 mg/kg. Metformin is dissolved in saline and is gavaged at a volume of 0.25-0.5 mL twice a day at an interval of 12 h, started immediately before NSC 37745 injection.

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.
    参考文献

    Metformin hydrochloride 相关分类

    完整储备液配制表

    * 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
    储备液的保存方式和期限：-80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)。-80°C储存时，请在6个月内使用，-20°C储存时，请在1个月内使用。

    可选溶剂 浓度 溶剂体积 质量 1 mg 5 mg 10 mg 25 mg
    DMSO / H2O 1 mM 6.0379 mL 30.1896 mL 60.3792 mL 150.9480 mL
    5 mM 1.2076 mL 6.0379 mL 12.0758 mL 30.1896 mL
    10 mM 0.6038 mL 3.0190 mL 6.0379 mL 15.0948 mL
    H2O 15 mM 0.4025 mL 2.0126 mL 4.0253 mL 10.0632 mL
    20 mM 0.3019 mL 1.5095 mL 3.0190 mL 7.5474 mL
    25 mM 0.2415 mL 1.2076 mL 2.4152 mL 6.0379 mL
    30 mM 0.2013 mL 1.0063 mL 2.0126 mL 5.0316 mL
    40 mM 0.1509 mL 0.7547 mL 1.5095 mL 3.7737 mL
    50 mM 0.1208 mL 0.6038 mL 1.2076 mL 3.0190 mL
    60 mM 0.1006 mL 0.5032 mL 1.0063 mL 2.5158 mL
    80 mM 0.0755 mL 0.3774 mL 0.7547 mL 1.8868 mL
    100 mM 0.0604 mL 0.3019 mL 0.6038 mL 1.5095 mL

    * 备注：如您选择水作为储备液，请稀释至工作液后，再用 0.22 μm 的滤膜过滤除菌后使用。

    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

    Keywords:

    Metformin1115-70-41,1-Dimethylbiguanide盐酸二甲双胍甲福明降糖片AMPKAutophagyMitophagyAMP-activated protein kinaseMitochondrial Autophagymitochondrialrespiratorychainliverinsulinsensitivitytype2diabetesblood-brainbarrierInhibitorinhibitorinhibit

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